Auntie, A.K.A. Experiment 516, is an illegal genetic experiment created by Jumba Jookiba. She is designed to mutate invertebrate lifeforms (does so by using her long tongue to mutate all types of invertebrates).
Experiment 516 was the 516th genetic experiment created by Jumba with Hämsterviel's funding. She was designed to eat all bugs up to make the bug population decrease.
516 and the other first 624 experiments were deactivated and smuggled to Earth by Jumba during his mission to capture Experiment 626.
All of the experiment pods were released and scattered across the island of Kauai.
At the unknown point, she was activated, captured, tamed and named Auntie.
Auntie is a brown anteater-like experiment.
The effects of the mutations normally result in excessive growth and aggressive behavior, her abilities also affect invertebrate experiments.